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g tead4 74  (Addgene inc)


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    Addgene inc g tead4 74
    G Tead4 74, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcmx+gal4+tead4/pCMX-Gal4-TEAD4+(Plasmid+%2333105)/pm41534572-42-12-16
    Average 93 stars, based on 9 article reviews
    g tead4 74 - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Reporter Assay:

    Article Title: Aurora A kinase activates YAP signaling in triple-negative breast cancer.
    Article Snippet: The Yes-associated protein (YAP) is an effector that transduces the output of the Hippo pathway to transcriptional modulation.. Considering the role of YAP in cancers, this protein has emerged as a key node in malignancy development.. In this study, we determined that Aurora A kinase acts as a positive regulator for YAP-mediated transcriptional machinery.

    Plasmid Preparation:

    Article Title: Aurora A kinase activates YAP signaling in triple-negative breast cancer.
    Article Snippet: The Yes-associated protein (YAP) is an effector that transduces the output of the Hippo pathway to transcriptional modulation.. Considering the role of YAP in cancers, this protein has emerged as a key node in malignancy development.. In this study, we determined that Aurora A kinase acts as a positive regulator for YAP-mediated transcriptional machinery.

    Article Title: Targeted degradation of specific TEAD paralogs by small molecule degraders
    Article Snippet: To make pTRE-Puro-pan-TEAD, the entire cassette containing all the TEAD was amplified from pCDNA3.1-pan-TEAD and cloned between MluI and AgeI in pTRE by NEBuilder® HiFi DNA Assembly. .. To make pGEX-3X-TEAD1-YBD-C359A, pGEX-3X-TEAD2-YBD-C380A, pGEX-3X-TEAD3-YBD-C368A, pGEX-3X-TEAD4-YBD-C367A, the coding sequences for YBD of TEAD1-4 were PCR amplified from pCMX-GAL4-TEAD1 (Addgene #33108), pCMX-GAL4-TEAD2 (Addgene #33107), pCMX-GAL4-TEAD3 (Addgene #33106), pCMX-GAL4-TEAD4 (Addgene #33105), pRK5-Myc-TEAD4 (Addgene #24638), as two overlapping fragments with the mutations introduced in the primers for amplifying them, and cloned into the BamH1 site in PGEX-3X plasmid by NEBuilder® HiFi DNA Assembly. ..

    Article Title: αE-catenin inhibits a Src–YAP1 oncogenic module that couples tyrosine kinases and the effector of Hippo signaling pathway
    Article Snippet: .. 8xGTIIC plasmid was generated by Dr. S. Piccolo(Dupont et al., 2011) and was obtained from Addgene (plasmid #34615). pUAS-LUC2 was generated by L. Luo (Potter et al., 2010) and obtained from Addgene (Plasmid #: 24343). pCMX-GAL4-TEAD4 was generated by Dr. K. Guan and obtained from Addgene (Plasmid #: 24640). .. CMV-Renilla-LUC was obtained from Promega. pFN19A-YAP2 was generated by PCR-amplifying YAP2 from GST-YAP2 (generated by K. Guan and obtained from Addgene (Plasmid #:24637)) with 5`-gccggcgatcg ccatggatcccgggcagcagccgccgcct-3` and 5`- aggggtttaaacctataaccatgtaagaaagctttctttatcta-3` and cloning using SgfI and PmeI.

    Article Title: A covalent inhibitor of the YAP–TEAD transcriptional complex identified by high-throughput screening
    Article Snippet: pCI-HA-YAP , YAP , N-Terminal HA , Addgene, plasmid #27007. .. pCMX-Gal4-TEAD4 , TEAD4 , N-Terminal Gal4 , Addgene, plasmid #33105. .. GAL4UAS-LUC Reporter , GAL4UAS , C-Terminal luciferase , Addgene, plasmid #64125.

    Article Title: c-Abl antagonizes the YAP oncogenic function
    Article Snippet: .. The GTIIC reporter plasmid was a kind gift of H Sasaki. pCMX-GAL4-TEAD4 was kindly provided by KL Guan (Addgene, plasmid no. 33105). ..

    Polymerase Chain Reaction:

    Article Title: Targeted degradation of specific TEAD paralogs by small molecule degraders
    Article Snippet: To make pTRE-Puro-pan-TEAD, the entire cassette containing all the TEAD was amplified from pCDNA3.1-pan-TEAD and cloned between MluI and AgeI in pTRE by NEBuilder® HiFi DNA Assembly. .. To make pGEX-3X-TEAD1-YBD-C359A, pGEX-3X-TEAD2-YBD-C380A, pGEX-3X-TEAD3-YBD-C368A, pGEX-3X-TEAD4-YBD-C367A, the coding sequences for YBD of TEAD1-4 were PCR amplified from pCMX-GAL4-TEAD1 (Addgene #33108), pCMX-GAL4-TEAD2 (Addgene #33107), pCMX-GAL4-TEAD3 (Addgene #33106), pCMX-GAL4-TEAD4 (Addgene #33105), pRK5-Myc-TEAD4 (Addgene #24638), as two overlapping fragments with the mutations introduced in the primers for amplifying them, and cloned into the BamH1 site in PGEX-3X plasmid by NEBuilder® HiFi DNA Assembly. ..

    Amplification:

    Article Title: Targeted degradation of specific TEAD paralogs by small molecule degraders
    Article Snippet: To make pTRE-Puro-pan-TEAD, the entire cassette containing all the TEAD was amplified from pCDNA3.1-pan-TEAD and cloned between MluI and AgeI in pTRE by NEBuilder® HiFi DNA Assembly. .. To make pGEX-3X-TEAD1-YBD-C359A, pGEX-3X-TEAD2-YBD-C380A, pGEX-3X-TEAD3-YBD-C368A, pGEX-3X-TEAD4-YBD-C367A, the coding sequences for YBD of TEAD1-4 were PCR amplified from pCMX-GAL4-TEAD1 (Addgene #33108), pCMX-GAL4-TEAD2 (Addgene #33107), pCMX-GAL4-TEAD3 (Addgene #33106), pCMX-GAL4-TEAD4 (Addgene #33105), pRK5-Myc-TEAD4 (Addgene #24638), as two overlapping fragments with the mutations introduced in the primers for amplifying them, and cloned into the BamH1 site in PGEX-3X plasmid by NEBuilder® HiFi DNA Assembly. ..

    Clone Assay:

    Article Title: Targeted degradation of specific TEAD paralogs by small molecule degraders
    Article Snippet: To make pTRE-Puro-pan-TEAD, the entire cassette containing all the TEAD was amplified from pCDNA3.1-pan-TEAD and cloned between MluI and AgeI in pTRE by NEBuilder® HiFi DNA Assembly. .. To make pGEX-3X-TEAD1-YBD-C359A, pGEX-3X-TEAD2-YBD-C380A, pGEX-3X-TEAD3-YBD-C368A, pGEX-3X-TEAD4-YBD-C367A, the coding sequences for YBD of TEAD1-4 were PCR amplified from pCMX-GAL4-TEAD1 (Addgene #33108), pCMX-GAL4-TEAD2 (Addgene #33107), pCMX-GAL4-TEAD3 (Addgene #33106), pCMX-GAL4-TEAD4 (Addgene #33105), pRK5-Myc-TEAD4 (Addgene #24638), as two overlapping fragments with the mutations introduced in the primers for amplifying them, and cloned into the BamH1 site in PGEX-3X plasmid by NEBuilder® HiFi DNA Assembly. ..

    Generated:

    Article Title: αE-catenin inhibits a Src–YAP1 oncogenic module that couples tyrosine kinases and the effector of Hippo signaling pathway
    Article Snippet: .. 8xGTIIC plasmid was generated by Dr. S. Piccolo(Dupont et al., 2011) and was obtained from Addgene (plasmid #34615). pUAS-LUC2 was generated by L. Luo (Potter et al., 2010) and obtained from Addgene (Plasmid #: 24343). pCMX-GAL4-TEAD4 was generated by Dr. K. Guan and obtained from Addgene (Plasmid #: 24640). .. CMV-Renilla-LUC was obtained from Promega. pFN19A-YAP2 was generated by PCR-amplifying YAP2 from GST-YAP2 (generated by K. Guan and obtained from Addgene (Plasmid #:24637)) with 5`-gccggcgatcg ccatggatcccgggcagcagccgccgcct-3` and 5`- aggggtttaaacctataaccatgtaagaaagctttctttatcta-3` and cloning using SgfI and PmeI.



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    mCMY020 inhibits YAP driven transcription in cells. (A) Relative luminance signal from TEAD-LUC MCF-7 cells treated with the indicated concentrations of mCMY020 for 24 hours ( n = 3, mean ± SEM). (B) Relative UAS-LUC signal from a <t>Gal4</t> assay with TEAD4 in HEK293T cells treated with the indicated concentrations of mCMY020 or verteporfin (VTP) for 24 hours ( n = 10, mean ± SEM). Significance was determined by Welch and Brown–Forsythe ANOVA; **** p < 0.0001. (C) Relative transcript levels of CYR61 , CTGF , and ANKRD1 from H226 cells treated with 10 μM mCMY020 for 24 hours. ( n = 3, mean ± SEM). Significance was determined by two-tailed t -test; *** p < 0.001. (D) Volcano plot of RNA-sequencing data depicting the fold change of transcripts in response to 24 hour treatment with mCMY020 (10 μM). (E) GSEA plots of YAP-dependent gene sets (curated from Zhao et al. and Cordenonsi et al. ) in response to mCMY020.
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    Figure 7: Compound 3.1 inhibits TEAD1, TEDA2, TEAD3 and <t>TEAD4</t> activity.
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    mCMY020 inhibits YAP driven transcription in cells. (A) Relative luminance signal from TEAD-LUC MCF-7 cells treated with the indicated concentrations of mCMY020 for 24 hours ( n = 3, mean ± SEM). (B) Relative UAS-LUC signal from a Gal4 assay with TEAD4 in HEK293T cells treated with the indicated concentrations of mCMY020 or verteporfin (VTP) for 24 hours ( n = 10, mean ± SEM). Significance was determined by Welch and Brown–Forsythe ANOVA; **** p < 0.0001. (C) Relative transcript levels of CYR61 , CTGF , and ANKRD1 from H226 cells treated with 10 μM mCMY020 for 24 hours. ( n = 3, mean ± SEM). Significance was determined by two-tailed t -test; *** p < 0.001. (D) Volcano plot of RNA-sequencing data depicting the fold change of transcripts in response to 24 hour treatment with mCMY020 (10 μM). (E) GSEA plots of YAP-dependent gene sets (curated from Zhao et al. and Cordenonsi et al. ) in response to mCMY020.

    Journal: RSC Chemical Biology

    Article Title: A covalent inhibitor of the YAP–TEAD transcriptional complex identified by high-throughput screening †

    doi: 10.1039/d3cb00044c

    Figure Lengend Snippet: mCMY020 inhibits YAP driven transcription in cells. (A) Relative luminance signal from TEAD-LUC MCF-7 cells treated with the indicated concentrations of mCMY020 for 24 hours ( n = 3, mean ± SEM). (B) Relative UAS-LUC signal from a Gal4 assay with TEAD4 in HEK293T cells treated with the indicated concentrations of mCMY020 or verteporfin (VTP) for 24 hours ( n = 10, mean ± SEM). Significance was determined by Welch and Brown–Forsythe ANOVA; **** p < 0.0001. (C) Relative transcript levels of CYR61 , CTGF , and ANKRD1 from H226 cells treated with 10 μM mCMY020 for 24 hours. ( n = 3, mean ± SEM). Significance was determined by two-tailed t -test; *** p < 0.001. (D) Volcano plot of RNA-sequencing data depicting the fold change of transcripts in response to 24 hour treatment with mCMY020 (10 μM). (E) GSEA plots of YAP-dependent gene sets (curated from Zhao et al. and Cordenonsi et al. ) in response to mCMY020.

    Article Snippet: pCMX-Gal4-TEAD4 , TEAD4 , N-Terminal Gal4 , Addgene, plasmid #33105.

    Techniques: Two Tailed Test, RNA Sequencing

    Journal: RSC Chemical Biology

    Article Title: A covalent inhibitor of the YAP–TEAD transcriptional complex identified by high-throughput screening †

    doi: 10.1039/d3cb00044c

    Figure Lengend Snippet:

    Article Snippet: pCMX-Gal4-TEAD4 , TEAD4 , N-Terminal Gal4 , Addgene, plasmid #33105.

    Techniques: Plasmid Preparation, Luciferase

    Figure 7: Compound 3.1 inhibits TEAD1, TEDA2, TEAD3 and TEAD4 activity.

    Journal: Journal of Medicinal Chemistry

    Article Title: Antiproliferative and Antimigratory Effects of a Novel YAP–TEAD Interaction Inhibitor Identified Using in Silico Molecular Docking

    doi: 10.1021/acs.jmedchem.8b01402

    Figure Lengend Snippet: Figure 7: Compound 3.1 inhibits TEAD1, TEDA2, TEAD3 and TEAD4 activity.

    Article Snippet: Plasmids expressing GAL4-fusions of TEAD1 (#33108), TEAD2 (#33107), TEAD3(#33106) and TEAD4 (#33105) and FLAG-YAP (#18881) were obtained from Addgene.

    Techniques: Activity Assay